Seminars

Seminars for the The Mass Spectrometry Interest Group of the NCI at Frederick will generally be scheduled for the 2nd Tuesday of each month for 2008. A seminar is usually a 45-50 minute presentation on a topic of general interest followed by open discussion.

Meetings

Separate from the seminars, there will also be smaller meetings which will consist of a 20-30 minute presentation on a topic of specific interest followed by open discussion. Meetings will not have a regular schedule.

Seminar

Speaker: Arie Hawkins, University of Maryland, Baltimore County, Baltimore, MD 21250

Topic: "Structural determination of regulatory domains present in retroviral RNA using FT-ICRMS"

Place: Building 549, Conference Room A, NCI at Frederick, Frederick, MD - Need Directions?

Time: Tuesday, April 22, 2008, at 2:00 PM

Abstract: RNA molecules can fold in very complex 3D structures that, due to their size and flexibility, may represent a challenge for NMR and X-ray crystallography. The problem is exacerbated in retroviruses by the fact that regulatory domains of the RNA genome can assume alternative conformations that mediate different functions of the viral lifecycle. Approaches based on chemical probing and MS detection (MS3D) have emerged as valid alternatives for the investigation of the structure and dynamics of functional RNA. Their development has taken advantage of the introduction of suitable software packages for translating the sparse distance constraints provided by MS3D into full-fledged, all-atom models. These approaches will be discussed using examples from HIV-1 and MMLV.

Paul Rudnick, National Institute of Standards and Technology, Gaithersburg, MD 20899
"Reference Libraries of Peptide Tandem Mass Spectra: 2007"
September 18, 2007

Yongdong Wang, Cerno Bioscience, Danbury, CT 06897
"Formula ID: Accurate Mass to Spectral Acuracy, FTMS to Quadrupole MS, and Internal Calibration to Self Calibration"
October 16, 2007

Jinshan Wang, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742
"Integration of O-18 Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins"
November 27, 2007

Ichiro Hirano, Shimadzu Corporation, Tokyo, Japan
"Component Identification Using a Quadrupole Ion Trap Time-of-Flight Mass Spectrometer"
January 29, 2008

Alice Delvolve, National Institute on Drug Abuse (NIDA), NIH, Baltimore, MD 21224
"Modeling of PNA-DNA Non-Covalent Complexes"
March 11, 2008

Arie Hawkins, University of Maryland, Baltimore County, Baltimore, MD 21250
"Structural determination of regulatory domains present in retroviral RNA using FT-ICRMS"
April 22, 2008

Speaker: To be announced
Topic: To be announced