Hay-Yan Jack Wang National Institute on Drug Abuse (NIDA) Office: 410-550-6870 extension 31 FAX: E-mail: hwang@intra.nida.nih.gov Job Title: Laboratory Manager Ph.D. in Physiology from the Johns Hopkins Bloomberg School of Public Health

Speaker: Hay-Yan Jack Wang, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224

Topic: Brain Lipidomics: A MALDI Mass Spectrometry Study and the Application of Related Techniques

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, September 13, 2005, at 2:00 PM

Abstract: Other than water and protein, lipids are the most abundant biomolecules in the brain. Lipids mediate a wide range of important biological functions, such as intracellular compartmentalization, membrane protein regulation, and signal transduction. Recent advancement in MALDI MS techniques made in situ analyses of lipids in the brain sections possible. This new technique overcomes the disadvantages of traditional extraction-based brain lipid studies by establishing the correlation between the distribution of lipid species and histology. The identified lipid species can be further confirmed by MS/MS. Overall, this new technique can rapidly and accurately study the tissue makeup with minimal amount of sample consumption and preparation as compared to the traditional extraction or immunohistochemistry methods. With modification in sample preparation, matrix selection and instrument tuning this technique can be applied to in situ analyses and localization of drugs, peptides and proteins.

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