Jeremy Lambert PhyNexus, Natick, MA Office: 408-267-7214 FAX: E-mail: jlambert@phynexus.com Job Title: Manager of Business Development B.S. in Biochemistry and Molecular Biology from the Boston University

Speaker: Jeremy Lambert, PhyNexus, Natick, MA

Topic: Micro-Scale Protein Enrichment and Purification Using New Column Technology

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, September 28, 2004, at 2:00 PM

Abstract: As the interest in characterizing protein interactions has increased, so has the number of high-throughput analytical technologies (microarrays, protein chips, mass spec) that offer the ability to study large numbers of samples in fine detail. However, as proteins are inherently complex molecules, elucidation of protein interactions requires that the samples for study are of sufficient purity and activity to produce reliable, high-quality data. Access to suitable expression systems for generating large collections of native and recombinant antibodies has enabled researchers to begin embarking on large-scale studies but a significant bottleneck exists for isolating and purifying proteins in scale and quantity that are required for such studies. A unique, yet simple process has been developed to purify and enrich antibodies or recombinant proteins containing affinity tags. Incorporating a new column technology expands the range of applications from simple low throughput to fully automated 96-well processes. These unique devices are capable of preparing concentrations of up to 5 mg/ml of purified proteins in small final volumes e.g. 10-20 ul, in less than 20 minutes.