Jack Henion Advion BioSciences, Inc. Office: 607-266-0665 FAX: E-mail: henionj@advion.com Job Title: Co-Founder and CEO Ph.D. in Analytical Chemistry from State University of NY at Albany

Speaker: Jack Henion, Advion BioSciences, Inc., Ithaca, NY

Topic: Chip-Based Nanoelectrospray: Applications in BioMarker Screening, Proteomics, Metabolite Identification, and Non Covalent Interactions

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Thursday, September 2, 2004, at 2:00 PM

Abstract: Mass spectrometry (MS) is an essential tool in modern life sciences research. Electrospray mass spectrometry has provided major advances for studies of biological importance with both small and large molecule compounds. This modern, versatile ionization mode coupled with on-line LC/MS and LC/MS/MS techniques has essentially revolutionized the bioanalytical sciences. There are new developments in electrospray ionization that can provide further analytical capabilities for the challenges presented by the trace determination of important biological compounds in complex samples. This lecture will describe a novel system that delivers this capability while complementing on-line HPLC techniques. Recently there have been reports on the miniaturization of analytical techniques. Although the promise of a true 'lab-on-a-chip' system has not yet enjoyed widespread commercialization, important developments are occurring that could lead to this capability. An important analytical opportunity exists if we apply miniaturization techniques to electrospray mass spectrometry. Hence, nanoelectrospray mass spectrometry via a miniaturized, chip-based system can provide automated high-quality, and facile mass spectrometric determination of small and large molecules isolated from biological samples. Nanoelectrospray is well-suited for studies of small molecule compounds isolated from biological samples as well as biologics in proteomics and related areas. Nanoelectrospray offers the benefits of improved sensitivity, reduced matrix suppression, comparable analyte response, a larger dynamic range, and minimum sample consumption. Examples will be shown for the characterization of drug metabolites, the identification of proteins via analysis of enzymatic digests of protein mixtures, the determination of glycosylation and phosphorylation sites in proteins, and studies of non covalent interactions. Each of these examples will employ automated nanoelectrospray infusion analyses. Finally, recent results from on-line nanoscale LC/MS/MS employing the ESI chip as the nanoelectrospray emitter will be described.