Jeffrey A. Kowalak National Institute of Mental Health Office: 301-496-4242 FAX: 301-480-0198 E-mail: jkowalak@intra.nimh.nih.gov Job Title: Staff Scientist Ph.D. in Biochemistry from the University of Utah

Speaker: Jeffrey A. Kowalak, Laboratory of Neurotoxicology, National Institute of Mental Health, NIH, Bethesda, MD 20892 USA

Topic: Evaluation of Microscale 2D HPLC-Tandem Mass Spectrometry for Functional Proteomics

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, September 10, 2002, at 2:00 PM

Abstract: Proteomics is rapidly evolving from analysis of global expression patterns toward characterization of highly purified protein complexes. Identification of interacting proteins is requisite for the elucidation of functional relationships. We have developed an automated system combining multi-dimensional chromatographic separation and mass spectrometric detection that can be used for both analysis of highly complex protein mixtures and comprehensive analysis of purified protein complexes. This 2D HPLC/MS system offers automated and high throughput analyses while retaining high sensitivity and highly reproducible performance. Results from the evaluation of our 2D system will be presented using the work of Wolters et al. [1] as a benchmark. Results from several multidimensional analyses will also be described.

[1] Dirk A. Wolters, Michael P. Washburn, and John R. Yates, III (2001) "An Automated Multidimensional Protein Identification Technology for Shotgun Proteomics" Analytical Chemistry, 73, 5683-5690.