Speaker: Haleem J. Issaq, SAIC Frederick, NCI-FCRDC, P.O. Box B, Frederick, MD

Topic: Capillary Electrophoresis: Recent Advances and Future Trends

Place: Building 426, Conference Room, NCI-Frederick, Frederick, MD

Time: Wednesday, April 21, 1999, at 1:00 PM

Abstract: Capillary electrophoresis (CE) is a micro separation technique which was introduced in 1967 by S. Hjerten. In 1981 J. Jorgenson introduced CE with on column detection. Today, most CE instrumentation is based on Jorgenson's design. In this presentation, we will review the most recent advances in CE instrumentation, detection, and applications. Capillary array electrophoresis which was first introduced by R. Mathies is today a viable commercial instrumentation with up to 96 capillaries. The effect of modifiers in both CE and micellar electrokinetic chromatography (MEKC) will be discussed. Also, the advantages and limitations of both capillary electrochromatography (CEC) and separations on a chip will be presented. Application of CE to DNA sequencing and the separation of DNA fragments, amino acids, peptides, polycyclic aromatic hydrocarbons, aflatoxins, etc., and the separation of enantiomers will be evaluated. A comparison of CE with HPLC, highlighting the advantages and limitations of both techniques will be made. Examples will be presented and future trends will be discussed.