Shelley N. Jackson NIDA, NIH Office: 410-550-6870 x24 FAX: E-mail: shjackson@intra.nih.gov Job Title: Post-Doctoral Fellow Ph.D. in Analytical Chemistry from Emory University

Speaker: Shelley N. Jackson, National Institute on Drug Abuse (NIDA), NIH, Baltimore, MD 21224

Topic: Phosphate and the Stabilization of Receptor-Receptor and Receptor-Ligand Interactions

Place: Building 549, Auditorium, Frederick National Laboratory for Cancer Research, Frederick, MD

Time: Tuesday, April 25, 2006, at 2:00 PM

Abstract: Protein-protein or peptide-peptide interactions are usually studied in the context of the lock and key model. We have found that linear interactions can play an important role in the formation of non-covalent complexes (NCX) in protein-protein as well as peptide-peptide interactions. Our previous work has highlighted the role of certain amino acid residues, mainly two or more adjacent arginine on one peptide and two or more adjacent glutamate, or aspartate, or a phosphorylated residue on the other in the formation of NCXs between peptides. In the present work, we employ mass spectrometry to investigate the gas-phase stability and dissociation pathways of NCXs formed between proteins, peptides, and lipids through an electrostatic interaction between and arginine residue and a phosphate group.