Speaker: Tina Gatlin, Large Scale Biology, Germantown, MD 20876 USA

Topic: A Highly-Automated Proteomics Facility: Merging 2D gel, Robotic Sample Prep, MS and Bioinformatics Technologies for Protein Identification and Characterization - Video (running time 01:05:20) ^*

Place: Building 426, Conference Room, NCI-Frederick, Frederick, MD

Time: Tuesday, February 12, 2002, at 2:00 PM

Abstract: An element to understanding a biological process is to identify proteins associated with that process. The combination of 2D gels and mass spectrometry are well suited for this task of protein identification. Additionally, quantitative information of protein abundances can further aid in understanding normal vs. perturbed systems. There are both gel-based and non-gel based methods for quantitation. While most of us would prefer to live in a "post-polyacrylamide world", we have overcome many of the traditional limitations of 2D-PAGE to develop a high-throughput system capable of high quality separations and quantitation. 2D gels are a key component to our ProGexTM platform which integrates technologies for enabling proteins within a sample to be prepared for analysis, separated, mapped, quantified and identified. The talk will focus mainly on automation methods for processing samples post-2D gel, including MS analysis by high-throughput MALDI-TOF and low-throughput LC-MS/MS. Work in progress to improve automation and future directions will also be discussed.

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