Robert J. Classon Shimadzu Scientific Instruments Office: 800-800-477-1227 x1906 FAX: E-mail: RJClasson@Shimadzu.com Job Title: B.Sc. in Chemistry and M.B.A. from the American University, Washington, DC

Speaker: Robert J. Classon, Shimadzu Scientific Instruments, Columbia, MD 21046

Topic: Everything You Wanted to Know About LC/MS

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, January 13, 2004, at 2:00 PM

Abstract: A short seminar on the "how to" of LC/MS and the things no one told you about and are not covered in books. The seminar has evolved from a short course given over the past few years at several technical meetings and covers the practical things people need to know in order to get successful results with LC/MS. There is no theory, no equations, and no lists of interfaces or hardware in this seminar, just practical information such as how to develop a LC/MS method (nearly everyone does it wrong but doesn't know it!). Issues will be covered such as how to improve sensitivity, how to increase the probability of finding your target on the first try, how to find solvents clean enough for LC/MS, how to determine the contaminants and artifacts and where they came from, how to do HPLC separations with fewer additives, how to reduce adduct formation, how to get better results for negative ions, and how to analyze difficult molecules at high sensitivity. A number of applications will be shown including some non-traditional approaches to analysis of anticancer drugs.

The revised slides to this seminar are in a 1.2 Megabyte PDF file, which can be opened and read by using the free Adobe Acrobat Reader®.